Bioroot RCS – is a paradigm shift for root canals possible?


Josette Camilleri discusses the composition and properties of using Bioroot RCS as a root canal sealer.


The introduction of hydraulic calcium silicate supplies to be used as root canal sealers was the final growth of the unique mineral trioxide combination (MTA) formulation.

The primary paper reported using MTA as a root canal sealer along side gutta-percha. Using MTA as a sealer resulted within the formation of mineralized tissues.

Thus, it was the primary examine to look into the method of biomineralisation and tissue reactions to the MTA and its calcium releasing potential. Using MTA as a sealer resulted in greater leakage apically than guttapercha obturations.

The mechanism of motion of MTA and its hydration mechanisms had been reported later. This was adopted by the event of economic root canal sealers. The primary ones in the marketplace had been developed in 2008 by Egeo and Angelus.

On the identical time, a paper about Proroot Endo sealer developed by Dentsply was additionally printed. Nevertheless, this sealer was not launched till lately in the marketplace.

The selection of sealers out there clinically up to now is proven within the desk beneath. Amongst these sealers is Bioroot RCS developed by Septodont. This text discusses the composition and properties of this sealer.

Josette Camilleri discusses the composition and properties of the use of Bioroot RCS as a root canal sealer.


The Bioroot RCS is the only formulation as proven within the desk. It’s water primarily based and the change from cement to sealer is dependent upon the inclusion of a water soluble polymer that enables materials circulation.

The primary use of a water soluble polymer added to Portland cement to enhance the fabric properties was printed in 2005. Using a water soluble polymer to create a root canal sealer was reported in 2009.

On this analysis the varied additions of polymer had been investigated and their impact on the resultant materials properties and hydration traits.

The addition of a water-soluble polymer to MTA didn’t alter the hydration traits of the fabric. This resulted in a cloth with improved properties appropriate to be used as endodontic sealer cement.

Displaying potential

Moreover, the novel sealer primarily based on MTA demonstrated ample setting time and was dimensionally steady. It had the potential for use as root canal sealer cement in scientific apply.

The Bioroot RCS is introduced in a powder and liquid format as proven in Determine 1. The powder consists of tricalcium silicate because the energetic cementitious materials and zirconium oxide radiopacifier.

Josette Camilleri discusses the composition and properties of the use of Bioroot RCS as a root canal sealer.
Determine 1: Bioroot RCS presentation by Septodont exhibiting the packaging with the container and scoop for the powder and liquid vials

The liquid consists of water, calcium chloride, povidone and a water soluble polymer. The sealer microstructure and elemental evaluation is proven in Determine 2A and 2C and its hydration over a interval of 28 days with the formation of the calcium hydroxide is proven in Determine 3.

The fundamental evaluation has been corroborated in one other current examine. When positioned in resolution, the sealer leaches excessive ranges of calcium ions when in comparison with different tricalcium silicate-based sealers equivalent to Endosequence BC sealer and MTA Fillapex.

Josette Camilleri discusses the composition and properties of the use of Bioroot RCS as a root canal sealer.
Determine 2A: Floor microstructure of Bioroot RCS exhibiting the primary phases current and elemental analyses proven in vitro
Determine 2B: Floor microstructure of Bioroot RCS in touch with the dentine indicating chemical modifications together with the formation phosphates


Setting time

The ultimate setting time of Bioroot RCS was proven to be 324 (±1) minutes which was shorter than that for AH Plus. MTA Fillapex didn’t set when used as a comparability to different tricalcium silicate-based root canal sealers.

The setting time of Bioroot RCS was lowered drastically on software of warmth utilized in heat vertical compaction obturation methods. The contact with a moist atmosphere lengthened the setting time significantly.

In truth, the producer recommends using Bioroot RCS with chilly obturation methods solely notably with gutta-percha in a single cone obturation approach.


Biooot RCS was proven to be much less soluble than AH Plus and MTA Fillapex instantly after immersion in water. Nevertheless, its solubility was greater over time when in comparison with the resinbased sealers.

The solubility enhances the organic properties of the sealer. Immersion in phosphate buffered saline improved the Bioroot RCS solubility in the long run and a floor precipitate was noticed after 14 and 28 days of immersion.

Circulation and movie thickness

Bioroot RCS reveals a decrease circulation and better movie thickness than the boundaries specified by ISO suggestions. The ISO suggestions are meant for inert sealers not like the Bioroot RCS.

The circulation and movie thickness are affected by the warmth utilized through the heat vertical compaction procedures. The producer the truth is recommends using chilly obturation methods.


The radiopacity of Bioroot RCS was proven to be higher than the decrease restrict specified by ISO and much like that of AH Plus and MTA Fillapex.

The radiopacity was proven to be about 9mm aluminium thickness. That is much like Endosequence BC sealer and better than for MTA Fillapex.

Calcium ion launch

Bioroot RCS was proven to launch excessive ranges of calcium in resolution.

That is a lot greater than different related sealer sorts. In truth, it releases double the quantity leached by Endosequence BC sealer and ten occasions as a lot as calcium ions launched by MTA Fillapex for a similar time intervals below the identical situations.

Biomineralization and the deposition of phosphates over the fabric when in touch with the dentine has been proven as indicated in Determine 2B and 2D.

Determine 2C: Floor microstructure of Bioroot RCS exhibiting the primary phases current and elemental analyses proven in vitro
Josette Camilleri discusses the composition and properties of the use of Bioroot RCS as a root canal sealer.
Determine 2D: Floor microstructure of Bioroot RCS in touch with the dentine indicating chemical modifications together with the formation phosphates


Contact of tricalcium silicate-based supplies with dentine and tissue fluids has been reported to result in the deposition of phosphates on the supplies floor. This has been extensively described for MTA.

The interplay of dentine and Bioroot RCS has been additionally effectively documented. A chemical bond is achieved by a mineral infiltration zone on the materials to tooth interface .

This property is necessary for sealers as bonding of the sealer to the basis canal dentine will result in much less microleakage. The mineral infiltration zone has been reported for Bioroot RCS utilizing confocal microscopy.

The mineral infiltration zone and the sealer tags guarantee sealer adaption and bonding to the basis canal dentine (Determine 4). The tags and mineral wealthy zone had been extra evident within the coronal portion than in mid-root and apically.

This may very well be brought on by restricted motion of the ethylene diamine tetracetic acid (EDTA) irrigation and removing of the smear layer additional down within the root canal.

The infiltration of phosphorus into the Bioroot RCS when this sealer is in touch with the dentine has not been confirmed. Floor part evaluation utilizing grazing angle X-Ray diffractometry didn’t discover the formation of calcium phosphate within the materials in touch with dentine.

When in comparison with MTA Fillapex and AH Plus, Bioroot RCS confirmed the best antimicrobial exercise. The basis canal sealers exerted a better antimicrobial exercise when EDTA was used as closing irrigant.

Sadly, the antimicrobial properties of the Bioroot RCS and different sealers of associated chemistry together with AH Plus confirmed a discount in antimicrobial properties when phosphate buffered saline was used as the ultimate irrigant throughout root canal remedy.

Determine 4: Confocal microscopic photos of Bioroot RCS interface with dentine at completely different ranges alongside the basis canal exhibiting the mineral infiltration zone and sealer tags in dentinal tubules


Elutions from Bioroot RCS and even direct seeding of cells over the supplies confirmed a excessive diploma of cell proliferation. Migration of periodontal ligament stem cells was noticed to be greater with Bioroot RCS and the cells maintained their mesenchymal phenotype.

This was corroborated by one other examine testing the elutions from Bioroot RCS and different tricalcium silicate-based sealers along with AH Plus.

The at some point materials elution confirmed no cytotoxic impact whereas 48 and 72 hour extracts exhibited gentle cytotoxicity. The at some point elution of Bioroot RCS was additionally evaluated in one other examine and no DNA double-strand breaks had been noticed in comparison with different resin- and silicate-based root canal sealers.

Bioroot RCS didn’t compromise the mineralization potential of pulpal A4 stem cells. It was not as cytotoxic as Pulp Canal Sealer which is a zinc oxide eugenol-based materials.

It didn’t recruit the pulpal stem cells towards differentiation however preserved their osteo-odontogenic intrinsic properties. Bioroot RCS additionally confirmed much less poisonous results on periodontal ligament cells than Pulp Canal Sealer and induced a better secretion of angiogenic and osteogenic progress elements than Pulp Canal Sealer.

Obturation with Bioroot RCS

Bioroot RCS was affected by the irrigation protocol used. Utilizing EDTA as the ultimate irrigant led to the discount of calcium releasing potential by half.

Moreover, in touch with dentine the calcium phosphate part was not fashioned when EDTA was used as closing irrigantas proven in Determine 5 which in contrast the crystalline phases fashioned after utilizing saline or EDTA as the ultimate irrigating resolution previous to obturation with Bioroot RCS.

Determine 5: X-ray diffraction plot of Bioroot RCS after contact with dentine irrigated with saline or EDTA exhibiting the depletion of calcium phosphate deposits on the fabric in touch with the dentine after irrigation with EDTA marked with black arrow

Irrigation with EDTA confirmed the very best antimicrobial properties for Bioroot RCS. The antimicrobial exercise of Bioroot RCS was considerably greater than that of MTA Fillapex and AH Plus.

Bioroot RCS confirmed the best antimicrobial exercise and this was enhanced by utilizing EDTA irrigating resolution. Using phosphate-rich irrigating options is contraindicated with Bioroot RCS and all tricalcium silicate-based sealers.

Utility of warmth throughout heat vertical compaction impacts the circulation and movie thickness of the Bioroot RCS. Thus, this sealer is really helpful to be used with single cone methods or lateral condensed gutta-percha. The selection of sealer ought to be thought-about when deciding on the obturation approach.

The producer recommends using single cone obturation approach with Bioroot RCS since this sealer is antimicrobial. Thus, its presence would probably get rid of any microorganisms left inside the basis canal house and within the dentinal tubules.

Its excessive antimicrobial exercise is clear and it’s nonetheless efficient regardless of the irrigating regime used.

The retreatability of Bioroot RCS sealer used along side gutta-percha in single cone obturation approach was higher in comparison with AH Plus as much less sealer remnants and shorter retreatment occasions had been noticed.


Bioroot RCS ought to be used along side a strong cone in any chilly obturation approach. The fabric solubility enhances the response of the fabric with environmental ionic trade thus favouring a organic response.

The Bioroot RCS is very antimicrobial and using EDTA enhances its antimicrobial exercise. This sealer was not developed to adapt to the classical suggestions of airtight seal. As a substitute, it goals to create an atmosphere inside the root canal that enhances organic exercise and maintains antimicrobial exercise.

Thus, a paradigm shift is feasible with Bioroot RCS.

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